Holographic Optical Tweezers (HOT) are powerful tools that can manipulate micro and nano-scale objects with high accuracy and precision. They are most commonly used for biological applications, such as cellular studies, and more recently, micro-structure assemblies. Automation has been of significant interest in the HOT field, since human-run experiments are time-consuming and require skilled operator(s). Automated HOTs, however, commonly use point traps, which focus high intensity laser light at specific spots in fluid media to attract and move micro-objects. In this paper, we develop a novel automated system of tweezing multiple micro-objects more efficiently using multiplexed optical traps. Multiplexed traps enable the simultaneous trapping of multiple beads in various alternate multiplexing formations, such as annular rings and line patterns. Our automated system is realized by augmenting the capabilities of a commercially available HOT with real-time bead detection and tracking, and wavefront-based path planning. We demonstrate the usefulness of the system by assembling two different composite micro-structures, comprising 5 $\mu m$ polystyrene beads, using both annular and line shaped traps in obstacle-rich environments.

Mapping traversal costs in an environment and planning paths based on this map are important for autonomous navigation. We present a neurobotic navigation system that utilizes a Spiking Neural Network Wavefront Planner and E-prop learning to concurrently map and plan paths in a large and complex environment. We incorporate a novel method for mapping which, when combined with the Spiking Wavefront Planner, allows for adaptive planning by selectively considering any combination of costs. The system is tested on a mobile robot platform in an outdoor environment with obstacles and varying terrain. Results indicate that the system is capable of discerning features in the environment using three measures of cost, (1) energy expenditure by the wheels, (2) time spent in the presence of obstacles, and (3) terrain slope. In just twelve hours of online training, E-prop learns and incorporates traversal costs into the path planning maps by updating the delays in the Spiking Wavefront Planner. On simulated paths, the Spiking Wavefront Planner plans significantly shorter and lower cost paths than A* and RRT*. The spiking wavefront planner is compatible with neuromorphic hardware and could be used for applications requiring low size, weight, and power.